L5178-S (LY-S) (ATCC® CRL-1723)

Organism: Mus musculus, mouse  /  Cell Type: chemically induced  /  Tissue: thymus  /  Disease: lymphoma

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Organism Mus musculus, mouse
Tissue thymus
Cell Type chemically induced
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain DBA/2
Storage Conditions liquid nitrogen vapor phase
Derivation This line was derived from the X-ray resistant L5178-R line (ATCC CRL-1722).

The parental cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse.
Tumorigenic Yes
Effects
Yes, in DBA/2 mice (but only weakly).
Comments The cells are sensitive to X-rays, ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD).
Complete Growth Medium Fischer's medium with 0.1 g/L sodium pyruvate and 1.125 sodium bicarbonate, 90%; horse serum, 10%
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 104 cells/mL and maintain between 5 x 103 and 7 x 105 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium, 90%; DMSO, 10%
Name of Depositor JZ Beer
References

Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899

Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332

Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899

Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332

Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312