L5178-R (LY-R) (ATCC® CRL-1722)

Organism: Mus musculus, mouse  /  Cell Type: chemically induced  /  Tissue: thymus  /  Disease: lymphoma

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Organism Mus musculus, mouse
Tissue thymus
Cell Type chemically induced
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain DBA/2
Applications
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.
The X-ray sensitive L5178-S line (ATCC CRL-1723) was derived from this line.
Tested and found negative for ectromelia virus.
Derivation
This cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse.
The X-ray sensitive L5178-S line (ATCC CRL-1723) was derived from this line.
Tumorigenic Yes
Effects
Yes, in DBA/2 mice
Comments
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD).
After 6 months to 3 years in culture, the line converts to an X-ray sensitive state.
Tested and found negative for ectromelia virus.
Complete Growth Medium Fischer's medium with 0.1 g/L sodium pyruvate and 1.125 g/L sodium bicarbonate, 90%; horse serum, 10%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 x 10 4 cells/mL and maintain between 3 x 10 4 and 7 x 10 4 cells/mL.
Medium Renewal: Twice per week
Cryopreservation
culture medium, 90%; DMSO, 10%
Name of Depositor JZ Beer
References

Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899

Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332

Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899

Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332

Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312