MOP-8 (ATCC® CRL-1709)

Organism: Mus musculus, mouse  /  Cell Type: SV40 transformed  / 

Organism Mus musculus, mouse
Cell Type SV40 transformed
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Age embryo
Strain NIH/Swiss
Applications
transfection host
Transfection host, especially for plasmids containing the polyomavirus origin of replication.
Derivation
The MOP-8 cell line was derived by transforming NIH 3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus.
Comments
The MOP-8 cell line was derived by transforming NIH 3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus.
The cells will support the replication of recombinant plasmids which contain the polyomavirus origin for DNA synthesis.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor JA Hassell
References

Muller WJ, et al. Isolation of large T antigen-producing mouse cell lines capable of supporting replication of polyomavirus-plasmid recombinants. Mol. Cell. Biol. 4: 2406-2412, 1984. PubMed: 6096696

Basic Documentation
References

Muller WJ, et al. Isolation of large T antigen-producing mouse cell lines capable of supporting replication of polyomavirus-plasmid recombinants. Mol. Cell. Biol. 4: 2406-2412, 1984. PubMed: 6096696