NFS-1.0 C-1 (ATCC® CRL-1705)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte; follicular center cell  /  Disease: lymphoma

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Organism Mus musculus, mouse
Cell Type B lymphocyte; follicular center cell
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain NFS.C58v-1
Applications
The NFS-1.0 C-1 line was derived from a spontaneous lymphoma.
The cells are also positive for ThB, 220), CD45R)] surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox).
Derivation
The NFS-1.0 C-1 line was derived from a spontaneous lymphoma.
Antigen Expression
CD5 +; CD32 +; CD44 +; CD45R +; ThB +; Lyb-2 +; Ia +
Genes Expressed
ecotropic retrovirus; mink cell focus forming retrovirus; immunoglobulin; lambda 3 light chain; the antibody reacts with bromelain treated mouse erythrocytes,CD5 +; CD32 +; CD44 +; CD45R +; ThB +; Lyb-2 +; Ia +
Cellular Products
ecotropic retrovirus; mink cell focus forming retrovirus; immunoglobulin; lambda 3 light chain; the antibody reacts with bromelain treated mouse erythrocytes
Comments
The NFS-1.0 C-1 line was derived from a spontaneous lymphoma.
The cells bear the characteristic B cell surface markers [(Ly 24, Pgp-1, CD44), (Ly 17, CD32), (Ly 1, CD5), (Lyb 2, Ly 5(B 5(B220), CD45R)].
The cells are also positive for ThB, 220), CD45R)] surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine and 0.05 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Atmosphere: 5% CO2 in air recommended
Isotype IgM
Name of Depositor WJ Davidson, HC Morse
References

Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201

Davidson WF. Immunologic abnormalities of the autoimmune mouse, Palmerston North. J. Immunol. 129: 751-758, 1982. PubMed: 6282970

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201

Davidson WF. Immunologic abnormalities of the autoimmune mouse, Palmerston North. J. Immunol. 129: 751-758, 1982. PubMed: 6282970