NFS-1.0 C-1 (ATCC® CRL-1705)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte; follicular center cell  /  Disease: lymphoma

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Cell Type B lymphocyte; follicular center cell
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain NFS.C58v-1
Applications
The NFS-1.0 C-1 line was derived from a spontaneous lymphoma.
The cells are also positive for ThB, 220), CD45R)] surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox).
Derivation
The NFS-1.0 C-1 line was derived from a spontaneous lymphoma.
Antigen Expression
CD5 +; CD32 +; CD44 +; CD45R +; ThB +; Lyb-2 +; Ia +
Genes Expressed
ecotropic retrovirus; mink cell focus forming retrovirus; immunoglobulin; lambda 3 light chain; the antibody reacts with bromelain treated mouse erythrocytes,CD5 +; CD32 +; CD44 +; CD45R +; ThB +; Lyb-2 +; Ia +
Cellular Products
ecotropic retrovirus; mink cell focus forming retrovirus; immunoglobulin; lambda 3 light chain; the antibody reacts with bromelain treated mouse erythrocytes
Comments
The NFS-1.0 C-1 line was derived from a spontaneous lymphoma.
The cells bear the characteristic B cell surface markers [(Ly 24, Pgp-1, CD44), (Ly 17, CD32), (Ly 1, CD5), (Lyb 2, Ly 5(B 5(B220), CD45R)].
The cells are also positive for ThB, 220), CD45R)] surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine and 0.05 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Atmosphere: 5% CO2 in air recommended
Isotype IgM
Name of Depositor WJ Davidson, HC Morse
References

Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201

Davidson WF. Immunologic abnormalities of the autoimmune mouse, Palmerston North. J. Immunol. 129: 751-758, 1982. PubMed: 6282970

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201

Davidson WF. Immunologic abnormalities of the autoimmune mouse, Palmerston North. J. Immunol. 129: 751-758, 1982. PubMed: 6282970