NFS-25 C-3 (ATCC® CRL-1695)

Organism: Mus musculus, mouse  /  Cell Type: pre-B lymphoblast  /  Disease: lymphoma

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Organism Mus musculus, mouse
Cell Type pre-B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, suspension
Biosafety Level 1
Disease lymphoma
Strain NFS.C58v-1
Applications
The NFS-25 C-3 cell line was derived from a spontaneous lymphoma.
They are negative for ThB, surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
The NFS-25 C-3 cell line was derived from a spontaneous lymphoma.
Antigen Expression
CD5 +; CD32 +; CD45R +
Genes Expressed
CD5 +; CD32 +; CD45R +
Comments
The NFS-25 C-3 cell line was derived from a spontaneous lymphoma.
The cells bear markers consistent with identification as large pre-B lymphoblasts [(Ly 17, CD32), (Ly 1, CD5), (Lyb 2, Ly 5(B220), CD45R)].
They are negative for ThB, surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 10 mM HEPES, 3 mM glutamine, 1 mM oxaloacetic acid, 0.2 units/ml insulin, 0.5 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol, 2 mcg/ml transferrin, 80%; NCTC medium 109, 10%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: 82.5% complete growth medium; 10% FBS; 7.5% DMSO.
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor WJ Davidson, HC Morse
References

Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201