NFS-5 C-1 (ATCC® CRL-1693)

Organism: Mus musculus, mouse  /  Cell Type: pre-B lymphoblast  /  Disease: lymphoma

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Organism Mus musculus, mouse
Cell Type pre-B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain NFS/N
Applications
The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus.
They are negative for ThB, surface immunoglobulin (sIg) and Ia.
Tested and found negative for ectromelia virus (mousepox).
Derivation
The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus.
Antigen Expression
CD5 +; CD32 +; CD45R +
Genes Expressed
ecotropic retrovirus; mink cell focus forming retrovirus,CD5 +; CD32 +; CD45R +
Cellular Products
ecotropic retrovirus; mink cell focus forming retrovirus
Comments
The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus.
The cells bear a pattern of markers consistent with identification as large pre-B lymphoblasts (Ly 17, CD32.
Ly 1, CD5.
Lyb 2, Ly 5(B220), CD45R).
They are negative for ThB, surface immunoglobulin (sIg) and Ia.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Modified Dulbecco's medium with 0.002 mg/ml transferrin and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor WJ Davidson, HC Morse
References

Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201