WEHI-274.1 (ATCC® CRL-1679)

Organism: Mus musculus, mouse  /  Cell Type: monocyte  /  Disease: Abelson murine leukemia virus-induced tumor

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Organism Mus musculus, mouse
Cell Type monocyte
Product Format frozen
Morphology monocyte
Culture Properties suspension
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Abelson murine leukemia virus-induced tumor
Strain BALB/c
Applications
The line expresses the MAC-1 antigen, does not express Ia and is not phagocytic.
Tested and found negative for ectromelia virus (mousepox).
Antigen Expression
MAC-1 antigen (CD11b)
Receptor Expression
Fc
Genes Expressed
MAC-1 antigen (CD11b)
Comments
The line expresses the MAC-1 antigen, does not express Ia and is not phagocytic.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; fetal bovine serum, 10%; 0.05 mM 2-mercaptoethanol
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Adherent cells can be dislodged by scraping. Start new cultures at 1 X 10 exp5 cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Name of Depositor NL Warner, LL Lanier
References

. Immunobiology and immunotherapy of cancer. New York: Elsevier/North Holland; 1979.

Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. Immunobiology and immunotherapy of cancer. New York: Elsevier/North Holland; 1979.

Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787