SJK-132-20 (ATCC® CRL-1640)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody neutralizes polymerase alpha activity, and reacts with polymerases from several species.
The cells need to be in culture for 6 to 7 days before antibody production is detectable.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Antigen Expression
H-2db
Genes Expressed
immunoglobulin; monoclonal antibody; against human DNA polymerase alpha,H-2db
Comments
Mice were immunized with fraction VIII human DNA polymerase alpha.
Alpha.
Spleen cells were fused with NS-1 myeloma cells.
The antibody neutralizes polymerase alpha activity, and reacts with polymerases from several species.
The cells need to be in culture for 6 to 7 days before antibody production is detectable.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC TIB-177.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor D Korn
References

Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672