SJK-132-20 (ATCC® CRL-1640)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody neutralizes polymerase alpha activity, and reacts with polymerases from several species.
The cells need to be in culture for 6 to 7 days before antibody production is detectable.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Antigen Expression
H-2db
Genes Expressed
immunoglobulin; monoclonal antibody; against human DNA polymerase alpha,H-2db
Cellular Products
immunoglobulin; monoclonal antibody; against human DNA polymerase alpha
Comments
Mice were immunized with fraction VIII human DNA polymerase alpha.
Alpha.
Spleen cells were fused with NS-1 myeloma cells.
The antibody neutralizes polymerase alpha activity, and reacts with polymerases from several species.
The cells need to be in culture for 6 to 7 days before antibody production is detectable.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC TIB-177.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor D Korn
References

Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672

Basic Documentation
References

Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672