3A-sub E [post crisis of 3A(tPA-30-1)] (ATCC® CRL-1584)

Organism: Homo sapiens, human  /  Cell Type: SV40 transformed  / 

Permits and Restrictions

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Organism Homo sapiens, human
Cell Type SV40 transformed
Product Format frozen
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Applications
This line was established from ATCC CRL-1583 cells that had begun to senesce.
Derivation
This line was established from ATCC CRL-1583 cells that had begun to senesce.
Genes Expressed
at 40C the cells produce human chorionic gonadotropin (hCG)
Comments
This line was established from ATCC CRL-1583 cells that had begun to senesce.
By passage 20 the cells had recovered and appeared capable of unlimited proliferation.
The saturation density (6 X 10 exp5 cells/sq cm) is increased and the morphology is altered relative to the parental line.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, rinse culture with fresh 0.25% trypsin-0.03% EDTA solution and let sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 9,12
D5S818: 11,13
D7S820: 10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,17
Name of Depositor MI Cour
Passage History
By passage 20 the cells had recovered and appeared capable of unlimited proliferation.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation