3197-3 (3T3-MSV) (ATCC® CRL-1568)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  /  Disease: sarcoma

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Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease sarcoma
Age embryo
Applications
The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus.
They do not produce virus particles.
Tested and found negative for ectromelia virus (mousepox).
Derivation
The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus.
Genes Expressed
The cells release a growth factor in serum free medium.
Comments
The cells were derived from ATCC CCL-92 by transformation with Moloney murine sarcoma virus.
They do not produce virus particles.
The cells have a tendency to slough off the flask surface.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 95%; bovine calf serum, 5%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.25% trypsin solution for 5 minutes, add fresh medium, aspirate and dispense into new flasks. Subculture every 3 to 6 days.
Name of Depositor JE DeLarco
References

de Larco JE, Todaro GJ. Growth factors from murine sarcoma virus-transformed cells. Proc. Natl. Acad. Sci. USA 75: 4001-4005, 1978. PubMed: 211512

Bassin RH, et al. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int. J. Cancer 6: 95-107, 1970. PubMed: 4319233

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

de Larco JE, Todaro GJ. Growth factors from murine sarcoma virus-transformed cells. Proc. Natl. Acad. Sci. USA 75: 4001-4005, 1978. PubMed: 211512

Bassin RH, et al. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int. J. Cancer 6: 95-107, 1970. PubMed: 4319233