NALM-1 (ATCC® CRL-1567)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast  /  Tissue: peripheral blood  /  Disease: Leukemia

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Leukemia
Age 3 years
Gender female
Applications
By means of a panel of specific xenoantisera, the NALM-1 cells were found to express a specific antigen of acute lymphoblastic leukemia and blast leukemia-associated antigen.
Furthermore, normal T-cell or B-cell antigens, detectable by the antisera used in this study, were not found in the NALM-1 line.
The cells exhibited no cell-surface receptors for sheep erythrocytes, IgG, or complement; neither cell-surface immunoglobulins nor cytoplasmic immunoglobulin were observed.
Storage Conditions liquid nitrogen vapor phase
Karyotype Contains Philadelphia chromosome (Ph1).
Derivation
A permanent hematopoietic cell line, designated NALM-1, was established from the peripheral blood of a patient who was in blastic crisis of Ph1-positive chronic myleocytic leukemia.
Clinical Data human
3 years
female
Comments
A permanent hematopoietic cell line, designated NALM-1, was established from the peripheral blood of a patient who was in blastic crisis of Ph1-positive chronic myleocytic leukemia. By means of a panel of specific xenoantisera, the NALM-1 cells were found to express a specific antigen of acute lymphoblastic leukemia and blast leukemia-associated antigen. The cells exhibited no cell-surface receptors for sheep erythrocytes, IgG, or complement; neither cell-surface immunoglobulins nor cytoplasmic immunoglobulin were observed. Furthermore, normal T-cell or B-cell antigens, detectable by the antisera used in this study, were not found in the NALM-1 line.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(5) viable cells/ml. Maintain cultures at a cell concentration between 1-2 X 10(5) and 1 X 10(6) cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Cryopreservation
Freeze medium: complete growth medium 95%,DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11,15
D5S818: 11
THO1: 7,9
TPOX: 9,11
vWA: 15,17
Name of Depositor J Minowada
References

Minawada J, et al. A non-T, non-B human leukemia cell line (NALM-1): establishment of the cell line and presence of leukemia-associated antigens. J. Natl. Cancer Inst. 59(1): 83-87, 1977. PubMed: 301573.

Sonta SI, et al. Cytogenetic study of a new Ph1-positive cell line (NALM-1). J. Natl. Cancer Inst. 59(3): 833-837, 1977. PubMed: 302341.

Pelz AF. Re-analysis of the cell line NALM-1 karyotype by GTG-banding, spectral karyotyping, and whole chromosome painting. Cancer Genet. Cytogenet. 156(1): 59-61, 2005. PubMed: 15588857.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Minawada J, et al. A non-T, non-B human leukemia cell line (NALM-1): establishment of the cell line and presence of leukemia-associated antigens. J. Natl. Cancer Inst. 59(1): 83-87, 1977. PubMed: 301573.

Sonta SI, et al. Cytogenetic study of a new Ph1-positive cell line (NALM-1). J. Natl. Cancer Inst. 59(3): 833-837, 1977. PubMed: 302341.

Pelz AF. Re-analysis of the cell line NALM-1 karyotype by GTG-banding, spectral karyotyping, and whole chromosome painting. Cancer Genet. Cytogenet. 156(1): 59-61, 2005. PubMed: 15588857.