NALM-1 (ATCC® CRL-1567)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast  /  Tissue: peripheral blood  /  Disease: Leukemia

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Organism Homo sapiens, human
Tissue
peripheral blood
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Leukemia
Age 3 years
Gender female
Applications
By means of a panel of specific xenoantisera, the NALM-1 cells were found to express a specific antigen of acute lymphoblastic leukemia and blast leukemia-associated antigen.
Furthermore, normal T-cell or B-cell antigens, detectable by the antisera used in this study, were not found in the NALM-1 line.
The cells exhibited no cell-surface receptors for sheep erythrocytes, IgG, or complement; neither cell-surface immunoglobulins nor cytoplasmic immunoglobulin were observed.
Storage Conditions liquid nitrogen vapor phase
Karyotype Contains Philadelphia chromosome (Ph1) [ Pubmed: 302341, 15588857].
Derivation
A permanent hematopoietic cell line, designated NALM-1, was established from the peripheral blood of a patient who was in blastic crisis of Ph1-positive chronic myleocytic leukemia.
Clinical Data
A permanent hematopoietic cell line, designated NALM-1, was established from the peripheral blood of a patient who was in blastic crisis of Ph1-positive chronic myleocytic leukemia.
female
Comments
A permanent hematopoietic cell line, designated NALM-1, was established from the peripheral blood of a patient who was in blastic crisis of Ph1-positive chronic myleocytic leukemia. By means of a panel of specific xenoantisera, the NALM-1 cells were found to express a specific antigen of acute lymphoblastic leukemia and blast leukemia-associated antigen. The cells exhibited no cell-surface receptors for sheep erythrocytes, IgG, or complement; neither cell-surface immunoglobulins nor cytoplasmic immunoglobulin were observed. Furthermore, normal T-cell or B-cell antigens, detectable by the antisera used in this study, were not found in the NALM-1 line. [Pubmed: 301573].
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(5) viable cells/ml. Maintain cultures at a cell concentration between 1-2 X 10(5) and 1 X 10(6) cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Cryopreservation
Freeze medium: complete growth medium 95%,DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11,15
D5S818: 11
THO1: 7,9
TPOX: 9,11
vWA: 15,17
Name of Depositor J Minowada
References

Minawada J, et al. A non-T, non-B human leukemia cell line (NALM-1): establishment of the cell line and presence of leukemia-associated antigens. J. Natl. Cancer Inst. 59(1): 83-87, 1977. PubMed: 301573.

Sonta SI, et al. Cytogenetic study of a new Ph1-positive cell line (NALM-1). J. Natl. Cancer Inst. 59(3): 833-837, 1977. PubMed: 302341.

Pelz AF. Re-analysis of the cell line NALM-1 karyotype by GTG-banding, spectral karyotyping, and whole chromosome painting. Cancer Genet. Cytogenet. 156(1): 59-61, 2005. PubMed: 15588857.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Minawada J, et al. A non-T, non-B human leukemia cell line (NALM-1): establishment of the cell line and presence of leukemia-associated antigens. J. Natl. Cancer Inst. 59(1): 83-87, 1977. PubMed: 301573.

Sonta SI, et al. Cytogenetic study of a new Ph1-positive cell line (NALM-1). J. Natl. Cancer Inst. 59(3): 833-837, 1977. PubMed: 302341.

Pelz AF. Re-analysis of the cell line NALM-1 karyotype by GTG-banding, spectral karyotyping, and whole chromosome painting. Cancer Genet. Cytogenet. 156(1): 59-61, 2005. PubMed: 15588857.