CCD-33Lu (ATCC® CRL-1490)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Tissue: lung  / 

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Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 7 years
Gender male
Ethnicity Caucasian
Derivation
The line was derived from normal lung tissue from a child who died of a brain tumor.
Clinical Data
male
Caucasian
7 years
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37°C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 11,13
THO1: 6,9.3
TPOX: 9,11
vWA: 15,16
Name of Depositor GP Maxwell
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation