G-361 (ATCC® CRL-1424)

Organism: Homo sapiens, human  /  Tissue: skin  /  Disease: malignant melanoma

Organism Homo sapiens, human
Tissue
skin
Product Format frozen
Morphology epithelial
Biosafety Level 1
Disease malignant melanoma
Age 31 years adult
Gender male
Ethnicity Caucasian
Clinical Data
31 years adult
Caucasian
male
Genes Expressed
melanin
Cellular Products
melanin
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA for several minutes, remove trypsin and let the culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 24 hours after seeding from a thawed ampule, then 2 to 3 times per week
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 15
D5S818: 11
D7S820: 8,11
THO1: 7,9
TPOX: 8,10
vWA: 15,17
Isoenzymes
G6PD, B
Name of Depositor PT Peebles
References

Peebles P, et al. Isolation of four unusual pediatric solid tumor cell lines. Pediatr. Res. 12: 485, 1978.

Basic Documentation
Other Documentation
References

Peebles P, et al. Isolation of four unusual pediatric solid tumor cell lines. Pediatr. Res. 12: 485, 1978.