ConA-B1-VICK (ATCC® CRL-12357)

Organism: Gallus gallus, chicken  /  Cell Type: T lymphocyte; transformed with reticuloendotheliosis virus (ATCC VR-770  / 

Organism Gallus gallus, chicken
Cell Type T lymphocyte; transformed with reticuloendotheliosis virus (ATCC VR-770
Product Format frozen
Morphology lymphoblast
Culture Properties clusters in suspension
Biosafety Level 2
Age 42 week old
Gender female
Applications
Two immortal cell lines were established, CON A-C1-VICK (ATCC CRL-12135) and ConA-B1-VICK (ATCC CRL-12357).
Both cell lines produce granulocyte colony stimulating factor (G-CSF).
When cultured in vitro, the cell lines produce and secrete immune lymphokines that may be administered to fowl to increase their resistance to infections.
The cell lines produce lymphokines which, following administration into either 18 day old chick embryos or day of hatch chicks, prevents extraintestinal Salmonella infection.
Neither the lymphokine or cell line induces viral pathogenesis in chickens.
For lymphokine production, remove cells from maintenance medium, wash in serum-free RPMI, culture at 5 x 10 exp6 cells/ml in serum-free RPMI containing 0.0075 mg/ml Concanavalin A (ConA) for 72 hours.
Derivation
Spleens were harvested and T cells were isolated.
Clinical Data
female
Genes Expressed
granulocyte colony stimulating factor (G-CSF)
Cellular Products
granulocyte colony stimulating factor (G-CSF)
Comments
Animals were immunized with Salmonella enteritidis.
Spleens were harvested and T cells were isolated.
T cells were incubated with Concanavalin A and subsequently transformed with Avian reticuloendotheliosis virus (REV-T with CSV)(ATCC VR-770).
Two immortal cell lines were established, CON A-C1-VICK (ATCC CRL-12135) and ConA-B1-VICK (ATCC CRL-12357).
Both cell lines produce granulocyte colony stimulating factor (G-CSF).
When cultured in vitro, the cell lines produce and secrete immune lymphokines that may be administered to fowl to increase their resistance to infections.
The cell lines produce lymphokines which, following administration into either 18 day old chick embryos or day of hatch chicks, prevents extraintestinal Salmonella infection.
Neither the lymphokine or cell line induces viral pathogenesis in chickens.
For lymphokine production, remove cells from maintenance medium, wash in serum-free RPMI, culture at 5 x 10 exp6 cells/ml in serum-free RPMI containing 0.0075 mg/ml Concanavalin A (ConA) for 72 hours.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor USDA/ARS
References

Kogut MH, et al. Method to produce granulocyte colony stimulating factor from immortalized avian T lymphocytes and method to produce immortalized cells. US Patent 5,691,200 dated Nov 25 1997

Basic Documentation
References

Kogut MH, et al. Method to produce granulocyte colony stimulating factor from immortalized avian T lymphocytes and method to produce immortalized cells. US Patent 5,691,200 dated Nov 25 1997