TK#1 [Embryonic stem cell line] (ATCC® CRL-11383™)

Organism: Mus musculus, mouse  /  Cell Type: embryonic stem cell

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Organism Mus musculus, mouse
Cell Type embryonic stem cell
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age embryo
Applications
The cell line was used to produce mutant mice with germ line transmission for the IRF-2 disruption.
Comments
The construct pMIRF2neoB was introduced into D3 embryonic stem (ES) cells by electroporation to disrupt the IRF-2 locus.
The cell line generated is deficient for the interferon regulatory factor 2 (IRF-2) gene.
The cell line was used to produce mutant mice with germ line transmission for the IRF-2 disruption.
Heterozygous mice were inbred to obtain mice homozygous for the disrupted gene.
The line should be grown on feeder layers of mitomycin C treated primary mouse embryonic fibroblasts or STO cells (see ATCC 56-X.2, MITC-STO cells).
Culture Conditions
Temperature: 37.0°C
Name of Depositor TW Mak
References

Mak TW, Taniguchi T. Mouse lacking the expression of interferon regulatory factor 2 (IRF-2). US Patent 5,675,059 dated Oct 7 1997

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Mak TW, Taniguchi T. Mouse lacking the expression of interferon regulatory factor 2 (IRF-2). US Patent 5,675,059 dated Oct 7 1997