TK#1 [Embryonic stem cell line] (ATCC® CRL-11383)

Organism: Mus musculus, mouse  /  Cell Type: embryonic stem cell  / 

Organism Mus musculus, mouse
Cell Type embryonic stem cell
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age embryo
Applications
The cell line was used to produce mutant mice with germ line transmission for the IRF-2 disruption.
Comments
The construct pMIRF2neoB was introduced into D3 embryonic stem (ES) cells by electroporation to disrupt the IRF-2 locus.
The cell line generated is deficient for the interferon regulatory factor 2 (IRF-2) gene.
The cell line was used to produce mutant mice with germ line transmission for the IRF-2 disruption.
Heterozygous mice were inbred to obtain mice homozygous for the disrupted gene.
The line should be grown on feeder layers of mitomycin C treated primary mouse embryonic fibroblasts or STO cells (see ATCC 56-X.2, MITC-STO cells).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Culture Conditions
Temperature: 37.0°C
Name of Depositor TW Mak
References

Mak TW, Taniguchi T. Mouse lacking the expression of interferon regulatory factor 2 (IRF-2). US Patent 5,675,059 dated Oct 7 1997

Basic Documentation
References

Mak TW, Taniguchi T. Mouse lacking the expression of interferon regulatory factor 2 (IRF-2). US Patent 5,675,059 dated Oct 7 1997