The construct pMIRF2neoB was introduced into D3 embryonic stem (ES) cells by electroporation to disrupt the IRF-2 locus.
The cell line generated is deficient for the interferon regulatory factor 2 (IRF-2) gene.
The cell line was used to produce mutant mice with germ line transmission for the IRF-2 disruption.
Heterozygous mice were inbred to obtain mice homozygous for the disrupted gene.
The line should be grown on feeder layers of mitomycin C treated primary mouse embryonic fibroblasts or STO cells (see ATCC 56-X.2
, MITC-STO cells).