(CRL-11383)

U.S. Patent Number
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The construct pMIRF2neoB was introduced into D3 embryonic stem (ES) cells by electroporation to disrupt the IRF-2 locus. 

The cell line generated is deficient for the interferon regulatory factor 2 (IRF-2) gene. 

The cell line was used to produce mutant mice with germ line transmission for the IRF-2 disruption. 

Heterozygous mice were inbred to obtain mice homozygous for the disrupted gene. 

The line should be grown on feeder layers of mitomycin C treated primary mouse embryonic fibroblasts or STO cells (see ATCC 56-X.2, MITC-STO cells).
Shipped frozen
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
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  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation
References

Mak TW, Taniguchi T. Mouse lacking the expression of interferon regulatory factor 2 (IRF-2). US Patent 5,675,059 dated Oct 7 1997

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm