9TR#1 [Embryonic stem cell line] (ATCC^® CRL-11379^™)

9TR#1 [Embryonic stem cell line] ATCC^® CRL-11379^™

frozen

For-Profit: $551.00 Non-Profit: $459.17

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Mus musculus, mouse
Cell Type	embryonic stem cell
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1
Age	embryo
Strain	129/Sv+c/+p
Applications	The cell line was used to produce mutant mice with germ line transmission for the TNFR disruption.

Comments	The vector gTR55 neoA was introduced into D3 embryonic stem (ES) cells by electroporation to disrupt the TNFR locus. The cell line generated is deficient for the tumor necrosis factor receptor (TNFR) p55 gene. The cell line was used to produce mutant mice with germ line transmission for the TNFR disruption. Heterozygous mice were inbred to obtain mice homozygous for the disrupted gene. The line should be grown on feeder layers of mitomycin C treated primary mouse embryonic fibroblasts or STO cells (see ATCC 56-X.2, MITC-STO cells).

Comments

The vector gTR55 neoA was introduced into D3 embryonic stem (ES) cells by electroporation to disrupt the TNFR locus.

The cell line generated is deficient for the tumor necrosis factor receptor (TNFR) p55 gene.

The cell line was used to produce mutant mice with germ line transmission for the TNFR disruption.

Heterozygous mice were inbred to obtain mice homozygous for the disrupted gene.

The line should be grown on feeder layers of mitomycin C treated primary mouse embryonic fibroblasts or STO cells (see ATCC 56-X.2, MITC-STO cells).

Complete Growth Medium	Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
Subculturing	Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks. A feeder layer must be used to grow these cells.
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C

Name of Depositor	TW Mak
References	Mak TW. Mutant mouse having a disrupted TNFRp55. US Patent 5,684,222 dated Nov 4 1997

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
References	Mak TW. Mutant mouse having a disrupted TNFRp55. US Patent 5,684,222 dated Nov 4 1997