D1.1 (ATCC® CRL-10915)

Organism: Homo sapiens, human  /  Cell Type: T lymphoblast  /  Disease: acute T cell leukemia

Organism Homo sapiens, human
Cell Type T lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute T cell leukemia
Gender male
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is derivative of Jurkat that is CD4 negative.
Clinical Data
male
Comments The cells have contact dependent helper activity.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 to 3 X 105 viable cells/mL.
Interval: Maintain cultures at cell concentrations between 2 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 8,11
D16S539: 10,11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18,19,20
Name of Depositor Trustees of Columbia University
References

Lederman S, et al. Murine monoclonal antibody (5c8) recognizes a human glycoprotein on the surface of T-lymphocytes, compositions containing same. US Patent 5,474,771 dated Dec 12 1995

. Protein recognized by an antibody that specifically binds an epitope that is specifically bound by monoclonal antibody 5c8. U.S. Patent 6,793,924 dated Sep 21 2004

Basic Documentation
References

Lederman S, et al. Murine monoclonal antibody (5c8) recognizes a human glycoprotein on the surface of T-lymphocytes, compositions containing same. US Patent 5,474,771 dated Dec 12 1995

. Protein recognized by an antibody that specifically binds an epitope that is specifically bound by monoclonal antibody 5c8. U.S. Patent 6,793,924 dated Sep 21 2004