JM1 (ATCC® CRL-10423)

Organism: Homo sapiens, human  /  Cell Type: pre-B lymphoblast  /  Disease: B cell lymphoma

Organism Homo sapiens, human
Cell Type pre-B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease B cell lymphoma
Applications
The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia.
The cells are negative for CD7, My 7, My 9 and surface IgM.
Storage Conditions liquid nitrogen vapor phase
Derivation
The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia. The cells are negative for CD7, My 7, My 9 and surface IgM.
Clinical Data
The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia.
Antigen Expression
HLA-DR; Homo sapiens, expressed
CD19; Homo sapiens, expressed
CD10; Homo sapiens, expressed
Genes Expressed
CD19; Homo sapiens, expressed ,CD10; Homo sapiens, expressed ,HLA-DR; Homo sapiens, expressed
Comments
The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia. The cells are negative for CD7, My 7, My 9 and surface IgM.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • fetal bovine serum to a final concentration of 10%

Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures may be established by centrifugation. Adherent cells can be dislodged by scraping. Initiate cultures at 2 to 5 x 10(5) viable cells/ml. Subculture when cell density reaches 1 X 10(6) cells/ml.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 95%; DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
D5S818: 12
D13S317: 11
D7S820: 8, 10
D16S539: 9
vWA: 17
THO1: 6
TPOX: 10, 11
CSF1PO: 12
Amelogenin: X Y
Name of Depositor Immunex Corporation
References

Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717

Basic Documentation
References

Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717