JM1 (ATCC® CRL-10423)

Organism: Homo sapiens, human  /  Cell Type: pre-B lymphoblast  /  Disease: B cell lymphoma

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Organism Homo sapiens, human
Cell Type pre-B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease B cell lymphoma
Applications
The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia.
The cells are negative for CD7, My 7, My 9 and surface IgM.
Storage Conditions liquid nitrogen vapor phase
Derivation
The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia. The cells are negative for CD7, My 7, My 9 and surface IgM.
Clinical Data
The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia.
Antigen Expression
HLA-DR; Homo sapiens, expressed
CD19; Homo sapiens, expressed
CD10; Homo sapiens, expressed
Genes Expressed
CD19; Homo sapiens, expressed ,CD10; Homo sapiens, expressed ,HLA-DR; Homo sapiens, expressed
Comments
The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia. The cells are negative for CD7, My 7, My 9 and surface IgM.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • fetal bovine serum to a final concentration of 10%

Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures may be established by centrifugation. Adherent cells can be dislodged by scraping. Initiate cultures at 2 to 5 x 10(5) viable cells/ml. Subculture when cell density reaches 1 X 10(6) cells/ml.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 95%; DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
D5S818: 12
D13S317: 11
D7S820: 8, 10
D16S539: 9
vWA: 17
THO1: 6
TPOX: 10, 11
CSF1PO: 12
Amelogenin: X Y
Name of Depositor Immunex Corporation
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
References

Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717