SW756 (ATCC® CRL-10302)

Organism: Homo sapiens, human  /  Disease: squamous cell carcinoma

Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease squamous cell carcinoma
Age 46 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 76; range = 60 to 96
Derivation
Temple Texas, United States
Clinical Data
female
Caucasian
46 years
Antigen Expression
HLA A1, A24, B8, B44, Cw2, Cx, DR6Y; Le3; Le4; Le5
Genes Expressed
HLA A1, A24, B8, B44, Cw2, Cx, DR6Y; Le3; Le4; Le5
Tumorigenic Yes
Effects
Yes, in nude mice
Virus Susceptibility Influenza A virus , Influenza A virus
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Protocol: Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 12,13
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 17
Isoenzymes
G6PD, B
GLO-I, 1-2
Name of Depositor Univ. Texas System, Board of Regents
Year of Origin 1974
References

Freedman RS, et al. Monoclonal antibody to a human MDR1 multidrug resistance gene product, and uses. US Patent 5,434,076 dated Jul 18 1995

Freedman RS, et al. Characterization of a cell line (SW756) derived from a human squamous carcinoma of the uterine cervix. In Vitro 18: 719-726, 1982. PubMed: 7129487

PR8/A/34

The SW756 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1974 from biopsy material from a poorly differentiated invasive squamous carcinoma of the uterine cervix. The patient had not received chemotherapy or radiation prior to the biopsy.

Basic Documentation
References

Freedman RS, et al. Monoclonal antibody to a human MDR1 multidrug resistance gene product, and uses. US Patent 5,434,076 dated Jul 18 1995

Freedman RS, et al. Characterization of a cell line (SW756) derived from a human squamous carcinoma of the uterine cervix. In Vitro 18: 719-726, 1982. PubMed: 7129487

PR8/A/34

The SW756 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1974 from biopsy material from a poorly differentiated invasive squamous carcinoma of the uterine cervix. The patient had not received chemotherapy or radiation prior to the biopsy.