XB-2 (ATCC® CL-177)

Organism: Mus musculus, mouse  /  Cell Type: epithelial keratinocyte  /  Disease: teratoma

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Organism Mus musculus, mouse
Cell Type epithelial keratinocyte
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Disease teratoma
Strain CXBGB/By
Applications
When cultured at low cell density as adherent cells, few cells differentiate.
When placed in suspension culture, however, cultures demonstrate colony forming ability decrease, nuclear pyknosis and other features of keratinocyte differentiation.
Storage Conditions liquid nitrogen vapor phase
Comments
XB-2 cells can undergo a process of terminal differention similar to keratinocytes. When cultured at low cell density as adherent cells, few cells differentiate. When placed in suspension culture, however, cultures demonstrate colony forming ability decrease, nuclear pyknosis and other features of keratinocyte differentiation.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Remove medium, add fresh trypsin (0.25%) - EDTA (0.53mM) solution for several minutes. Remove and place culture at room temperature for 5-10 minutes until the cells detach. Add fresh medium, aspirate and dispense into new culture flask containing MITC-STO fibroblasts (ATCC 56-X.2). The XB-2 cells will not survive without a feeder system.
Subcultivation Ratio: See subcultivation information above
Medium Renewal: Once per week
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: This cell line requires a feeder layer of irradiated mouse fibroblasts when starting from a thawed ampule and at subculture. THE CELLS WILL NOT SURVIVE WITHOUT A FEEDER LAYER.
Name of Depositor Massachusetts Institute of Technology
References

Green H, Rheinwald JG. Process for serially culturing keratinocytes. US Patent 4,016,036 dated Apr 5 1977

Morrissey JH, Green H. Differentiation-related death of an established keratinocyte line in suspension culture. J. Cell. Physiol. 97: 469-476, 1978. PubMed: 730781

Stevens LC. The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev. Biol. 21: 364-382, 1970. PubMed: 5436899

XB-2 was isolated from a transplantable mouse teratoma (No. 69691) established by L. C. Stevens.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Green H, Rheinwald JG. Process for serially culturing keratinocytes. US Patent 4,016,036 dated Apr 5 1977

Morrissey JH, Green H. Differentiation-related death of an established keratinocyte line in suspension culture. J. Cell. Physiol. 97: 469-476, 1978. PubMed: 730781

Stevens LC. The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev. Biol. 21: 364-382, 1970. PubMed: 5436899

XB-2 was isolated from a transplantable mouse teratoma (No. 69691) established by L. C. Stevens.