Jiyoye [Jijoye, P-2003, P-3J] (ATCC® CCL-87)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Disease: Burkitt's lymphoma

Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS
Disease Burkitt's lymphoma
Age 7 years
Gender male
Ethnicity Black, African
Applications
The cells carry and produce Epstein-Barr virus (EBV).
Clinical Data
male
Black, African
7 years
Antigen Expression
HLA A32, B17, Bw37
Genes Expressed
Epstein-Barr virus (EBV),HLA A32, B17, Bw37
Cellular Products
Epstein-Barr virus (EBV)
Virus Resistance
The cells are partially resistant to poliovirus and vesicular stomatitis viruses.
Comments
The cells carry and produce Epstein-Barr virus (EBV).
EBNA positive.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 to 6 X 10 exp5 viable cells/ml.
A maximum of 2 - 3 X 10 exp6 viable cells/ml is obtainable.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19
Isoenzymes
G6PD, B
Name of Depositor W Henle
References

. . J. Clin. Pathol. 38: 261, 1985.

Kohn G, et al. Involvement of C group chromosomes in five Burkitt lymphoma cell lines. J. Natl. Cancer Inst. 38: 209-222, 1967. PubMed: 6019732

. . In Vitro 4: 165, 1969.

Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082

Basic Documentation
References

. . J. Clin. Pathol. 38: 261, 1985.

Kohn G, et al. Involvement of C group chromosomes in five Burkitt lymphoma cell lines. J. Natl. Cancer Inst. 38: 209-222, 1967. PubMed: 6019732

. . In Vitro 4: 165, 1969.

Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082