Detroit 525 (ATCC® CCL-65)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 3.5 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype number = 45 plus centric fragment; XO; Turner syndrome.
Clinical Data
female
Caucasian
3.5 years
Virus Susceptibility Human poliovirus 1
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,13
D5S818: 11,13
D7S820: 12
THO1: 9.3
TPOX: 8
vWA: 17,18
Isoenzymes
G6PD, B
Name of Depositor CS Stulberg
Year of Origin October, 1963
References

Hart ZH, et al. A sex chromatin negative individual with chromosomes (XO) plus a persistent centric fragment. J. Pediatr. 66: 120-123, 1965. PubMed: 14253578

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hart ZH, et al. A sex chromatin negative individual with chromosomes (XO) plus a persistent centric fragment. J. Pediatr. 66: 120-123, 1965. PubMed: 14253578