Dempsey (ATCC® CCL-28)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 2.5 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype model number = 49; range = 41 to 95 Stability graph shows 100% stability in the stemline number (49). Karyotypes of cells with stemline number of chromosomes were stable with all cells having three extra chromosomes in the X, 6-12 group.
Clinical Data
male
Caucasian
2.5 years
Virus Susceptibility Human poliovirus 1
Comments
Klinefelter Syndrome - XXXXY.
Senesces after approximately 30 passages.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,12
THO1: 8,9.3
TPOX: 8
vWA: 17,18
Isoenzymes
G6PD, B
Name of Depositor TC Hsu
Passage History
Senesces after approximately 30 passages.
Year of Origin April, 1964
References

Hwu TC, Lockhart LH. The beginning and the terminal stages of DNA synthesis of human cells with an XXXXY constitution. Hereditas 52: 320-324, 1965. PubMed: 5889982

Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hwu TC, Lockhart LH. The beginning and the terminal stages of DNA synthesis of human cells with an XXXXY constitution. Hereditas 52: 320-324, 1965. PubMed: 5889982

Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475