WISH (ATCC^® CCL-25^™)

WISH ATCC^® CCL-25^™

frozen

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Derivation	This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
Markers	Y
Genes Expressed	The cells are positive for keratin by immunoperoxidase staining. The cells and tumors formed from the cells are positive for Dopa decarboxylase and are negative for the TAG-72 antigen.
Comments	Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination

Complete Growth Medium	Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing	Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37°C

STR Profile	Amelogenin: X CSF1PO: 9,10 D13S317: 13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18
Isoenzymes	G6PD, A

Name of Depositor	L Hayflick
References	Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous cultivation. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490 . . Am. J. Dis. Child. 103: 460, 1962.

Name of Depositor

L Hayflick

References

Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous cultivation. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490

. . Am. J. Dis. Child. 103: 460, 1962.

Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Additional Information
References	Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous cultivation. Exp. Cell Res. 23: 14-20, 1961. PubMed: 13712490 . . Am. J. Dis. Child. 103: 460, 1962.

Basic Documentation