SW1417 [SW-1417] (ATCC® CCL-238)

Organism: Homo sapiens, human  /  Tissue: colon  /  Disease: Dukes' type C, grade III, colorectal adenocarcinoma

Organism Homo sapiens, human
Tissue colon
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Dukes' type C, grade III, colorectal adenocarcinoma
Age 53 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data
female
53 years
Although the patient from which this cell was derived was blood type B, the cell line does not react with anti type B antibody despite the presence of normal levels of alpha1,3-galactosyltransferase activity.
Caucasian
Antigen Expression
blood type B; Rh+
Oncogene myc +; ras +; fos +; sis +; p53 -; myb -; abl -; ros -; src -
Genes Expressed
carcinoembryonic antigen (CEA) 362 ng/106 cells/10 days; keratin
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Comments

Although the patient from which this cell was derived was blood type B, the cell line does not react with anti type B antibody despite the presence of normal levels of alpha1,3-galactosyltransferase activity.
N-myc, K-ras, N-ras and myb oncogene expression were not detected.
The cells are positive for keratin by immunoperoxidase staining.
The line is positive for expression of c-myc, H-ras, sis and fos oncogenes.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 1 to 2 times per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 100%
Isoenzymes
ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1
Name of Depositor A Leibovitz
References

Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345

Basic Documentation
Other Documentation
References

Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345