COLO 205 (ATCC® CCL-222™)

Organism: Homo sapiens, human  /  Tissue: colon; derived from metastatic site: ascites  / 

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Organism Homo sapiens, human
Tissue
colon; derived from metastatic site: ascites
Product Format frozen
Morphology epithelial
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease Dukes' type D, colorectal adenocarcinoma
Age 70 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic.
Derivation
This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen.
Clinical Data 70 years
Caucasian
male
The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen.
Genes Expressed
carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10),The cells are positive for keratin by immunoperoxidase staining.
Tumorigenic Yes
Effects
Yes, in nude mice
(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Comments

The line was derived from tissue from the same patient as COLO 201 (ATCC CCL-224).

The cells are CSAp negative (CSAp-).

The cells are positive for keratin by immunoperoxidase staining.

COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.

This cell line is tumorigenic in mice. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Shake flask, transfer floating cells inot a new flask. Cells remaining attached may be removed using a standard trypsinization protocol. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 10,12
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
THO1: 8,9
TPOX: 11
vWA: 15
Isoenzymes
ES-D, 1-2
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1-2
PGM3, 1-2
Name of Depositor GE Moore
References

Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251

Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Bjork P, et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed: 7693697

Cross References

Nucleotide (GenBank) : AB012142 Homo sapiens hCAP1a mRNA for mRNA capping enzyme, complete cds.

Nucleotide (GenBank) : AB012143 Homo sapiens hCAP1b mRNA for mRNA capping enzyme, complete cds.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cancer cell line mutation data
References

Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251

Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Bjork P, et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed: 7693697