COLO 205 (ATCC® CCL-222)

Organism: Homo sapiens, human  /  Tissue: colon; derived from metastatic site: ascites  /  Disease: Dukes' type D, colorectal adenocarcinoma

Organism Homo sapiens, human
Tissue colon; derived from metastatic site: ascites
Product Format frozen
Morphology epithelial
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease Dukes' type D, colorectal adenocarcinoma
Age 70 years
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic.
Derivation
This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen.
Clinical Data 70 years
Caucasian
male
The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen.
Genes Expressed
carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10),The cells are positive for keratin by immunoperoxidase staining.
Tumorigenic Yes
Effects
Yes, in nude mice
Comments

The line was derived from tissue from the same patient as COLO 201 (ATCC CCL-224).

The cells are CSAp negative (CSAp-).

The cells are positive for keratin by immunoperoxidase staining.

COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.

 

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cells grow loosely attached and in suspension.

Shake flask, transfer floating cells into a new flask. Cells remaining attached may be removed using a standard trypsinization protocol. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which, contains trypsin inhibitor.
  2. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  3. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  4. Add appropriate aliquots of the cell suspension to new culture vessels.
  5. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 10,12
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
THO1: 8,9
TPOX: 11
vWA: 15
Isoenzymes
ES-D, 1-2
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1-2
PGM3, 1-2
Name of Depositor GE Moore
References

Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251

Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Bjork P, et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed: 7693697

Cross References

Nucleotide (GenBank) : AB012142 Homo sapiens hCAP1a mRNA for mRNA capping enzyme, complete cds.

Nucleotide (GenBank) : AB012143 Homo sapiens hCAP1b mRNA for mRNA capping enzyme, complete cds.

Basic Documentation
Other Documentation
FAQ's
  1. CCL-222 growth and morphology


    Date Updated: 2/21/2014

References

Semple TU, et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251

Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Bjork P, et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed: 7693697