CCD-25Lu (ATCC® CCL-215)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Disease: Glioma

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Glioma
Age 7 years
Gender male
Ethnicity Caucasian
Applications
The line was established from the lung of a patient who died of glioma of the brain stem.
Storage Conditions liquid nitrogen vapor phase
Karyotype normal male; diploid; stable
Derivation
The line was established from the lung of a patient who died of glioma of the brain stem.
Clinical Data
The line was established from the lung of a patient who died of glioma of the brain stem.
male
Caucasian
7 years
Comments
The line was established from the lung of a patient who died of glioma of the brain stem.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Interval: Subculture every 7 to 9 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,14
D16S539: 9,11
D5S818: 12
D7S820: 8,11
THO1: 6,7
TPOX: 11,12
vWA: 17,18
Name of Depositor S Dilworth
Year of Origin March, 1979