Hs888Lu (ATCC® CCL-211™) Organism: Homo sapiens, human / Cell Type: fibroblast General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Homo sapiens, human Cell Type fibroblast Product Format frozen Morphology fibroblast Culture Properties adherent Biosafety Level 1 Disease Osteosarcoma Age 20 years Gender male Ethnicity Caucasian Applications The line was derived from normal tissue from patient who had osteosarcoma metastatic to the lung. Storage Conditions liquid nitrogen vapor phase Karyotype normal male; diploid; stable Derivation The line was derived from normal tissue from patient who had osteosarcoma metastatic to the lung. Clinical Data The line was derived from normal tissue from patient who had osteosarcoma metastatic to the lung.maleCaucasian20 years Comments The line was derived from normal tissue from patient who had osteosarcoma metastatic to the lung.The tissue was taken from a normal area of lung. Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommendedMedium Renewal: Every 2 to 3 days Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37.0°CAtmosphere: air, 95%; carbon dioxide (CO2), 5% STR Profile Amelogenin: X,YCSF1PO: 10,11D13S317: 11,14D16S539: 8,12D5S818: 13D7S820: 7,11THO1: 10,9.3TPOX: 8,11vWA: 15,18 Name of Depositor WA Nelson-Rees Year of Origin August, 1975 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS