HeLa 229 (ATCC® CCL-2.1™) Organism: Homo sapiens, human / Tissue: cervix / General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Homo sapiens, human Tissue cervix Product Format frozen Morphology epithelial Culture Properties adherent (The line can be readily adapted to growth in suspension) Biosafety Level 2 [Cells contain human papilloma virus (HPV-18)] Disease adenocarcinoma Age 31 years Gender female Ethnicity Black Storage Conditions liquid nitrogen vapor phase Clinical Data 31 yearsBlackfemale HeLa Markers Y Virus Susceptibility Human adenovirus 7 Vesicular stomatitis virus Virus Resistance Human poliovirus 1 Human poliovirus 2 Human poliovirus 3 Comments Relatively resistant to polioviruses. The cells are positive for keratin by immunoperoxidase staining. This cell line can be readily adapted to growth in suspension. ATCC confirmed this cell line is positive for the presence of Papillomavirus viral DNA sequences via PCR. Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Cryopreservation Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSOStorage Temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: Air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C STR Profile Amelogenin: XCSF1PO: 9,10D13S317: 12,13.3D16S539: 9,10D5S818: 11,12D7S820: 8,12THO1: 7TPOX: 8,12vWA: 16,18 Isoenzymes G6PD, A Name of Depositor JT Syverton References Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 The line can be readily adapted to growth in suspension Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 The line can be readily adapted to growth in suspension