LA-4 (ATCC® CCL-196)

Organism: Mus musculus, mouse  /  Cell Type: urethane induced  /  Tissue: lung  /  Disease: adenoma

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Organism Mus musculus, mouse
Tissue lung
Cell Type urethane induced
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenoma
Age 28 weeks
Strain A/He
Storage Conditions liquid nitrogen vapor phase
Karyotype Unstable karyotype. Range = 38 to 256. Metacentric chromosomes are noted in about 12% of the cells. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor GD Stoner
References

Stoner GD, et al. Clonal isolation of epithelial cells from mouse lung adenoma. Cancer Res. 35: 2177-2185, 1975. PubMed: 167947

Stoner GD, et al. Epithelial cells cultured from mouse lung adenoma: biosynthesis of lecithin and comparison of phospholipids with the lung. Exp. Mol. Pathol. 29: 102-114, 1978. PubMed: 581072

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Stoner GD, et al. Clonal isolation of epithelial cells from mouse lung adenoma. Cancer Res. 35: 2177-2185, 1975. PubMed: 167947

Stoner GD, et al. Epithelial cells cultured from mouse lung adenoma: biosynthesis of lecithin and comparison of phospholipids with the lung. Exp. Mol. Pathol. 29: 102-114, 1978. PubMed: 581072