XC (ATCC® CCL-165)

Organism: Rattus norvegicus, rat  /  Cell Type: virus induced  /  Disease: Rous sarcoma

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Organism Rattus norvegicus, rat
Cell Type virus induced
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Rous sarcoma
Strain White-Weston
Storage Conditions liquid nitrogen vapor temperature
Virus Resistance
poliovirus 1
Comments
Syncytial plaques are formed when cells are infected with MuLV.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor JW Hartley
References

Rowe WP, et al. Plaque assay techniques for murine leukemia viruses. Virology 42: 1136-1139, 1970. PubMed: 4099080

Klement V, et al. Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture. Proc. Natl. Acad. Sci. USA 63: 753-758, 1969. PubMed: 4186808

. . Folia Biol. 8: 221-228, 1962.

. . Folia Biol. 7: 46-60, 1961.

. . Folia Biol. 9: 77-81, 1963.

. . Nature 186: 980-981, 1960.

Simkovic D, et al. An in vitro system for the detection of the Rous sarcoma virus in the cells of the rat tumour XC. Neoplasma 9: 104-106, 1962. PubMed: 13913043

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rowe WP, et al. Plaque assay techniques for murine leukemia viruses. Virology 42: 1136-1139, 1970. PubMed: 4099080

Klement V, et al. Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture. Proc. Natl. Acad. Sci. USA 63: 753-758, 1969. PubMed: 4186808

. . Folia Biol. 8: 221-228, 1962.

. . Folia Biol. 7: 46-60, 1961.

. . Folia Biol. 9: 77-81, 1963.

. . Nature 186: 980-981, 1960.

Simkovic D, et al. An in vitro system for the detection of the Rous sarcoma virus in the cells of the rat tumour XC. Neoplasma 9: 104-106, 1962. PubMed: 13913043