XC (ATCC® CCL-165)

Organism: Rattus norvegicus, rat  /  Cell Type: virus induced  /  Disease: Rous sarcoma

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Organism Rattus norvegicus, rat
Cell Type virus induced
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Rous sarcoma
Strain White-Weston
Storage Conditions liquid nitrogen vapor temperature
Virus Resistance
poliovirus 1
Comments
Syncytial plaques are formed when cells are infected with MuLV.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor JW Hartley
References

Rowe WP, et al. Plaque assay techniques for murine leukemia viruses. Virology 42: 1136-1139, 1970. PubMed: 4099080

Klement V, et al. Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture. Proc. Natl. Acad. Sci. USA 63: 753-758, 1969. PubMed: 4186808

. . Folia Biol. 8: 221-228, 1962.

. . Folia Biol. 7: 46-60, 1961.

. . Folia Biol. 9: 77-81, 1963.

. . Nature 186: 980-981, 1960.

Simkovic D, et al. An in vitro system for the detection of the Rous sarcoma virus in the cells of the rat tumour XC. Neoplasma 9: 104-106, 1962. PubMed: 13913043

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rowe WP, et al. Plaque assay techniques for murine leukemia viruses. Virology 42: 1136-1139, 1970. PubMed: 4099080

Klement V, et al. Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture. Proc. Natl. Acad. Sci. USA 63: 753-758, 1969. PubMed: 4186808

. . Folia Biol. 8: 221-228, 1962.

. . Folia Biol. 7: 46-60, 1961.

. . Folia Biol. 9: 77-81, 1963.

. . Nature 186: 980-981, 1960.

Simkovic D, et al. An in vitro system for the detection of the Rous sarcoma virus in the cells of the rat tumour XC. Neoplasma 9: 104-106, 1962. PubMed: 13913043