MOPC-31C (ATCC® CCL-130)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Disease: plasmacytoma; myeloma

Permits and Restrictions

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Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Strain BALB/cJ
Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Antigen Expression
H-2d
Genes Expressed
immunoglobulin,H-2d
Virus Resistance
poliovirus 3
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Leibovitz's L-15 medium with 2mM L-glutamine, 80%; fetal bovine serum, 20%

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Cultures can be maintained by addition of fresh medium, or by collecting cells as a pellet after centrifugation.
The packed cells are then resuspended in fresh medium.
The initial inoculum should not be less than 2 to 3 X 10 exp5 viable cells/ml.
A maximum of 1 to 2 X 10 exp6 cells/ml are obtainable.
Do not aspirate suspension to break up clumped cells.
Cryopreservation
Freeze medium: 90% FBS,10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor RJ Klebe
References

Yosida TH, et al. Chromosomal alteration and development of tumors. XIX. Chromosome constitution of tumor cells in 16 plasma cell neoplasms of BALB/c mice. J. Natl. Cancer Inst. 41: 1083-1097, 1968. PubMed: 4176942

Potter M, Robertson CL. Development of plasma-cell neoplasms in BALB/c mice after intraperitoneal injection of paraffin-oil adjuvant, heart-killed Staphylococcus mixtures. J. Natl. Cancer Inst. 25: 847-861, 1960. PubMed: 13737512

Potter M, Kuff EL. Disorders in the differentiation of protein secretion in neoplastic plasma cells. J. Mol. Biol. 9: 537-544, 1964. PubMed: 14202284

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Yosida TH, et al. Chromosomal alteration and development of tumors. XIX. Chromosome constitution of tumor cells in 16 plasma cell neoplasms of BALB/c mice. J. Natl. Cancer Inst. 41: 1083-1097, 1968. PubMed: 4176942

Potter M, Robertson CL. Development of plasma-cell neoplasms in BALB/c mice after intraperitoneal injection of paraffin-oil adjuvant, heart-killed Staphylococcus mixtures. J. Natl. Cancer Inst. 25: 847-861, 1960. PubMed: 13737512

Potter M, Kuff EL. Disorders in the differentiation of protein secretion in neoplastic plasma cells. J. Mol. Biol. 9: 537-544, 1964. PubMed: 14202284