Aedes albopictus (Mosquito larva) (ATCC® CCL-126)

Organism: Aedes albopictus, mosquito, Asian tiger  / 

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Organism Aedes albopictus, mosquito, Asian tiger
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age larva
Applications
transfection host
Storage Conditions liquid nitrogen vapor phase
Karyotype Karyotype stable within diploid stemline number. The chromosomes have median centromeres. In a few cases, a chromosome with a submedian centromere was noted. Two (2) cells with chromosome breaks (3%) and 6 cells with secondary constrictions (10%).
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Virus Susceptibility Dengue virus type 1
Dengue virus type 2
Dengue virus type 3
Colorado tick fever virus
Dengue virus type 4
Japanese encephalitis virus , Japanese encephalitis virus
Human poliovirus 1
Human herpesvirus 1 , Herpes simplex virus 1
Vaccinia virus
Vesicular stomatitis virus
Complete Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing
Protocol:
  1. Remove culture medium to a centrifuge tube.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin, 0.53 mM EDTA in Cell Dissociation Buffer Enzyme-free, Hanks’ based solution (GIBCO cat# 13150-016) solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 3.0 to 5.0 mL of Trypsin-Buffer solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. To remove trypsin-Buffer solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 x g for 5 to10 minutes.
  6. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 7 to 8 x 103 viable cells/cm2 is recommended. Maintain cultures at a cell density between 1 x 104 and 1 x 105 cells/cm2.
  7. Incubate cultures at 28°C in 95% air, 5% CO2
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 1 to 2 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 28.0°C; (Max. 28.0 °C, Min. 26.0°C)
Population Doubling Time 26
Name of Depositor SM Buckley
References

Singh KRP. Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36: 506-508, 1967.

Singh KRP, Paul SD. Multiplication of arboviruses in cell lines from from Aedes albopictus and Aedes aegypti. Curr. Sci. 37: 65-67, 1968.

Singh KR. Propagation of arboviruses in Singh's Aedes cell lines. I. Growth of arboviruses in Aedes albopictus and A. aegypti cell lines. Curr. Top. Microbiol. Immunol. 55: 127-133, 1971. PubMed: 5142320

Mitsuhashi J, Maramorosch K. Leafhopper tissue culture: Embryonic, nymphal and imaginal tissues from aseptic insects. Contrib. Boyce Thompson Inst. 22: 435-460, 1964.

Buckley SM. Susceptibility of the Aedes albopictus and A. aegypti cell lines to infection with arboviruses. Proc. Soc. Exp. Biol. Med. 131: 625-630, 1969. PubMed: 5787147

Yunker CE, Cory J. Colorado tick fever virus: growth in a mosquito cell line. J. Virol. 3: 631-632, 1969. PubMed: 5798247

tissue from freshly hatched larvae

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Singh KRP. Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36: 506-508, 1967.

Singh KRP, Paul SD. Multiplication of arboviruses in cell lines from from Aedes albopictus and Aedes aegypti. Curr. Sci. 37: 65-67, 1968.

Singh KR. Propagation of arboviruses in Singh's Aedes cell lines. I. Growth of arboviruses in Aedes albopictus and A. aegypti cell lines. Curr. Top. Microbiol. Immunol. 55: 127-133, 1971. PubMed: 5142320

Mitsuhashi J, Maramorosch K. Leafhopper tissue culture: Embryonic, nymphal and imaginal tissues from aseptic insects. Contrib. Boyce Thompson Inst. 22: 435-460, 1964.

Buckley SM. Susceptibility of the Aedes albopictus and A. aegypti cell lines to infection with arboviruses. Proc. Soc. Exp. Biol. Med. 131: 625-630, 1969. PubMed: 5787147

Yunker CE, Cory J. Colorado tick fever virus: growth in a mosquito cell line. J. Virol. 3: 631-632, 1969. PubMed: 5798247

tissue from freshly hatched larvae