IgH-2 (ATCC® CCL-108)

Organism: iguana  / 

Organism iguana
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age immature
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 35; range = 30 to 64.
The karyotype is stable. There is one large marker chromosome with a terminal centromere appearing in all cells in addition to 22 minute chromosomes, 10 large chromosomes with a median centromere and 2 large chromosomes with a submedian centromere.
Clinical Data
male
Virus Susceptibility iguana
Herpes simplex virus
Pseudorabies virus
Vaccinia virus
Human poliovirus 1
Vesicular stomatitis virus
Complete Growth Medium Eagles's Minimum Essential Medium with Hanks BSS, 2 mM L-glutamine, and 0.1 mM non-essential amino acids supplemented with 10% fetal bovine serum
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 36°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Incubate cultures at 36°C in a closed flask without CO2 in air atmosphere.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 36°C
Name of Depositor HF Clark
Year of Origin August, 1966
References

Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904

Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147

Cross References

Nucleotide (GenBank) : L27530 Iguana small cytoplasmic RNA (Y3).

Nucleotide (GenBank) : L27537 Iguana small cytoplasmic RNA (Y4).

Nucleotide (GenBank) : L27532 Iguana small cytoplasmic RNA (Y3) gene.

Nucleotide (GenBank) : L27534 Iguana small cytoplasmic RNA (Y4) gene.

Nucleotide (GenBank) : L27536 Iguana small cytoplasmic RNA (Y4) gene.

Basic Documentation
References

Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904

Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147