A6 (ATCC® CCL-102)

Organism: Xenopus laevis, frog, South African clawed  /  Tissue: kidney  / 

Permits and Restrictions

View Permits

Organism Xenopus laevis, frog, South African clawed
Tissue
kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender male
Clinical Data
male
Virus Resistance
poliovirus 1; vesicular stomatitis (Indiana); herpes simplex; vaccinia; pseudorabies
Complete Growth Medium NCTC 109 medium, 75%; distilled water, 15%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 26°C
Name of Depositor KA Rafferty
References

Mizell, M. Biology of amphibian tumors. New York: Springer-Verlag; 1969.

Rokaw MD, et al. Regulation of a sodium channel-associated G-protein by aldosterone. J. Biol. Chem. 271: 4491-4496, 1996. PubMed: 8626803

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mizell, M. Biology of amphibian tumors. New York: Springer-Verlag; 1969.

Rokaw MD, et al. Regulation of a sodium channel-associated G-protein by aldosterone. J. Biol. Chem. 271: 4491-4496, 1996. PubMed: 8626803