A6 (ATCC® CCL-102)

Organism: Xenopus laevis, frog, South African clawed  /  Tissue: kidney  / 

Permits and Restrictions

View Permits

Organism Xenopus laevis, frog, South African clawed
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype Hyperdiploid stemline of 44 chromosomes. Three extra chromosomes in median and submedian group plus 5 extra chromosomes not displayed in normal diploid cells. All cells with one or more chromosomes with secondary constrictions (frequently in N1 chromosomes). Some cells with fragments. Marker chromosomes differing from normal diploid types are: two small chromosomes with submedian centromeres; two small chromosomes with terminal centromeres; and one large chromosome with terminal centromere with a secondary constriction.
Derivation

The A6 cell line was initiated in July, 1965 by primary cultivation of normal kidneys from an adult male frog by K.A. Rafferty, Jr. (Biology of Amphibian Tumors, edited by M. Mizell, pp. 52-81, Springer-Verlag, 1969). 

Clinical Data
male
Virus Susceptibility Frog virus 4 , Frog virus 4
Virus Resistance
poliovirus 1; vesicular stomatitis (Indiana); herpes simplex; vaccinia; pseudorabies
Complete Growth Medium NCTC 109 medium, 75%; distilled water, 15%; fetal bovine serum, 10%
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Remove the trypsin, add fresh culture medium, aspirate and dispense into new flasks. Incubate at 26°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 26°C
Name of Depositor KA Rafferty
Passage History

A culture in the 59th serial passage was submitted to the American Type Culture Collection in December 1967.

References

Mizell, M. Biology of amphibian tumors. New York: Springer-Verlag; 1969.

Rokaw MD, et al. Regulation of a sodium channel-associated G-protein by aldosterone. J. Biol. Chem. 271: 4491-4496, 1996. PubMed: 8626803

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mizell, M. Biology of amphibian tumors. New York: Springer-Verlag; 1969.

Rokaw MD, et al. Regulation of a sodium channel-associated G-protein by aldosterone. J. Biol. Chem. 271: 4491-4496, 1996. PubMed: 8626803