pYMh1*81 (ATCC® 99601)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: BR Olsen, R Haronen

Permits and Restrictions

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Designations pYMh1*81
GenBank Number

X54413

Species Homo sapiens, human
Depositors BR Olsen, R Haronen
Vector
Construct size (kb): 3.200000047683716
Insert
DNA: cDNA
Insert lengths(kb): 0.2020000070333481
Tissue: chondrocytes
Gene product: collagen, type IX, alpha 1 [COL9A1]
Insert Size (kb) 0.202
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.2; SmaI--3.2; PvuII--2.5, 0.7.
Insert contains the following restriction sites (approximate kb from the 5' end): SmaI--0.15, 0.17.
References

Muragaki Y, et al. The complete primary structure of two distinct forms of human alpha1(IX) collagen chains. Eur. J. Biochem. 192: 703-708, 1990. PubMed: 2209617

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