2158e3 (ATCC® 99565)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: J Flint

Permits and Restrictions

View Permits

Designations 2158e3
Species Homo sapiens, human
Depositors J Flint
Applications
contains sequence useful for DNA diagnostics DNA Segment, unique subtelomeric region
probe for fluorescence in situ hybridization (FISH) (unlabelled) DNA Segment, unique subtelomeric region
Vector
Construct size (kb): 46.0
Insert
DNA: genomic
Insert lengths(kb): 40.0
Gene product: DNA Segment, unique subtelomeric region
Target Gene: DNA Segment, unique subtelomeric region
Insert Size (kb) 40.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
One of a set of unique probes (ATCC 99554-99594) for human chromosome subtelomeric regions. The probes are useful for fluorescence in situ hybridization (FISH), specifically in the detection and diagnosis of cryptic deletions and translocations.
Subcloned from yRM2158, available as ATCC 99420.
References

Sullivan DJ Jr., et al. A complete set of human telomeric probes and their clinical application. National Institutes of Health and Institute of Molecular Medicine collaboration. Nat. Genet. 14: 86-89, 1996. PubMed: 8782825

Product Sheet
Product Sheet
Product Sheet