pAQP1 Xenopus expression vector [UTR-01749, WI-9113, pCHIPev/BSII KS-] (ATCC® 99538)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: P Agre, GM Preston

Permits and Restrictions

View Permits

Designations pAQP1 Xenopus expression vector [UTR-01749, WI-9113, pCHIPev/BSII KS-]
GenBank Number

M77829

Species Homo sapiens, human
Depositors P Agre, GM Preston
Applications
construct to generate mRNA that can be expressed in Xenopus oocytes aquaporin 1 (channel-forming integral protein, 28 kD)
Vector
Construct size (kb): 4.057000160217285
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS-
Intact vector size: 2.961
Type of vector: phagemid
Vector end: HindIII
Vector end: PstI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->, 3-459
primer site: M13-20, ->
promoter for in vitro transcription: T7, ->
insert detection: lacZ', <-, 816-938
MCS: SacI...KpnI, ->, 657-759
promoter for in vitro transcription: T3, <-
primer site: reverse, <-
replicon: pMB1, 1032-1972
marker(s): ampR, <-, 1975-2832
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: AQP1
Other gene symbols: CHIP28
Gene name: aquaporin 1 (channel-forming integral protein, 28 kD)
Contains complete coding sequence?: Y
Chromosome: 7; Localization: 7 p14; 7, 84.8799999 cR
Tissue: bone marrow
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: PstI
Insert size (kb): 1.114
Cross references: DNA Seq. Acc.: M77829
Nucleotides 57-904 of the insert correspond to
nucleotides ~1-860 of M77829.
Insert lengths(kb): 1.113999962806702
Tissue: bone marrow
Gene product: aquaporin 1 (channel-forming integral protein, 28 kD) [AQP1]
Target Gene: aquaporin 1 (channel-forming integral protein, 28 kD)
Insert Size (kb) 1.114
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglI--1.5, 1.3, 1.1, 0.22; HindIII/PstI--2.8, 1.2; PvuII--2.4, 0.78, 0.6, 0.22.
Insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.08, 0.27; PvuII--0.34, 0.53.
The AQP1 coding sequence is flanked by 56 bp 5' untranslated sequence and 210 bp 3' untranslated sequence from the Xenopus beta-globin gene.
Sense mRNA can be synthesized in vitro from the SmaI digested contruct using T3 RNA polymerase. Resulting mRNA can be injected into and expressed in Xenopus oocytes.
References

Preston GM, Agre P. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991. PubMed: 1722319

Preston GM, et al. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 256: 385-387, 1992. PubMed: 1373524

Peter Agre, personal communication

Product Sheet
Product Sheet
Product Sheet