lambda92 [GC.92] (ATCC® 95667)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations lambda92 [GC.92]
GenBank Number

L10641

Species Homo sapiens, human
Vector
Construct size (kb): 48.79999923706055
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 17.85
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~1-17850 of the insert correspond to
nucleotides ~19800-37650 of L10641.
Insert lengths(kb): 17.85000038146973
Tissue: fetal liver (ATCC 37333)
Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) [GC]
Insert Size (kb) 17.850
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11, 6.2, 3.5, 2.7, 2.5, 1.9, 1.15; HindIII--18 (doublet), 6.2, 2.4, 1.4, 1.1, 1, 0.4; BamHI--14 (doublet), 8, 6.2, 4, 1.5, 0.5; SstI-->23, 8.5, 4.2;
BglII--18, 5.2, 4.85, 4.7, 3.7, 2.2, 2.1, 1.9, 1.4, 1.0, 0.9, 0.8, 0.75, 0.65, 0.4, 0.3.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.9, 1.05, 2.95, 5.45, 11.65, 14.35; SacI--2.4; HindIII--8.1, 10.4, 11.5, 12.8, 13.8, 14.2.
Begins in intron 2 and extends into intron 11.
ATCC 95664/95665 and 95666/95667 overlap by 6.45 kb and share EcoRI fragments of the following sizes (kb): 0.15, 1.9, 2.5.
ATCC 95666/95667 and 95668/95669 overlap by 7.8 kb and share an EcoRI fragment of 2.7 kb.
Contains Alu repeats, Kpn repeats, and a THE repeat.
References

Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650

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