pCu426CTR1-CYC1 (ATCC® 87738)

Applications: cloning vector  /  Depositors: S Labbe, DJ Thiele

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Designations pCu426CTR1-CYC1
Depositors S Labbe, DJ Thiele
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.3379998207092290
Vector: pCu426CTR1-CYC1 (phagemid)
Promoters: Promoter for expression CTR1-CYC1
Construction: pRS426, CTR1-CYC1 promoter
Construct size (kb): 6.337999820709229
Features: marker(s): URA3
marker(s): ampR
promoter for expression: CTR1-CYC1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1
cloning vector
Restriction digests of the vector give the following sizes (kb): SacI--6.4; SacI/SpeI--6.0, 0.38.
CTR3 and CTR1 are the copper transporter promoters and are repressed by the presence of copper and highly activated under copper-starvation conditions. The time course of copper-starvation activation and copper repression of a reporter gene is rapid.

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

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