pRep(A) [pREP] (ATCC® 87702)

Applications: vector for production of recombinant retrovirus  /  Depositors: H Iba

Permits and Restrictions

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Designations pRep(A) [pREP]
Depositors H Iba
Biosafety Level 1
Vector Information
Size (kb): 9.1999998092651370
Vector: pRep(A) (plasmid)
Construction: RSV (5' LTR, gag, pol, 5' env), pBR322
Marker(s):ampR
Construct size (kb): 9.199999809265137
Features: marker(s): ampR
other: 5' LTR
replicon: pMB1
restriction site: SalI
coding sequence: 5' env
coding sequence: gag
coding sequence: pol(derived from the Bryan strain of RSV
Applications
vector for production of recombinant retrovirus
Comments
Restriction digests of the clone give the following sizes (kb): EcoRV - 9.4; NdeI - 9.4; SalI - 9.4.
The parental plasmid pSR-REP which encodes pol, derived from the Schmidt-Ruppin subgroup A strain of RSV, was described in Cell 34: 597-607, 1983.
Rous sarcoma virus (RSV) derived vector (5' LTR, gag, pol and 5' env), for ligation with avian retroviral expression vectors (ATCC 87699 - 87701) to create proviral DNA capable of producing replication competent recombinant virus.
The vector can be linearized with SalI for ligation to one of the RSV-derived expression vectors.
The pol sequence is derived from the Bryan strain of RSV and is more efficient in virus production after transfection.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Murakami M, et al. High-level expression of exogenous genes by replication-competent retrovirus vectors with an internal ribosomal entry site. Gene 202: 23-29, 1997. PubMed: 9427541

Iba H, et al. Transforming potential and growth stimulating activity of the v-fos and c-fos genes carried by avian retrovirus vectors. Oncogene Res. 2: 121-133, 1988. PubMed: 2851123

Iba H, et al. Rous sarcoma virus variants that carry the cellular src gene instead of the viral src gene cannot transform chicken embryo fibroblasts. Proc. Natl. Acad. Sci. USA 81: 4424-4428, 1984. PubMed: 6087323

Related Products
component of:ATCC 87703
Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid

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