pRS405 (ATCC® 87516)

Applications: YI-type (integrating) shuttle vector  /  Depositors: P Hieter

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Designations pRS405
Depositors P Hieter
Biosafety Level 1
Host
Shipped in: E. coli DH5alpha
Vector Information
Size (kb): 5.507
Vector: pRS405 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Marker(s):LEU2,ampR
Construct size (kb): 5.507
Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
Applications
YI-type (integrating) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): ClaI--3.95, 1.55; BglI--3.8, 1.7; PvuII--5.0, 0.45.
A YI-type shuttle vector with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568

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component of:ATCC 87533
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Shipped frozen
Shipping Information

 Freeze dried phagemid in E. coli

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