p424 TEF (ATCC® 87365)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p424 TEF
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 6.2490000724792480
Vector: p424 TEF (plasmid)
Promoters: Promoter for expression TEF
Construction: pRS424
Marker(s):TRP1,ampR
Construct size (kb): 6.249000072479248
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: TEF
replicon: 2 micron
MCS: XhoI...XbaI
terminator: CYC1
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.2; EcoRI--6.2; SacI/XbaI--3.2, 1.7, 1.0, 0.44.
High copy number shuttle expression vector.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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