p70 [W3110/p70] (ATCC® 69334)

Organism: Clostridium histolyticum (Weinberg and Seguin) Bergey et al.  /  Clone Type: Clone  /  Depositors: H Lin, MS Nasoff, H Lin

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Designations p70 [W3110/p70]
Species Clostridium histolyticum (Weinberg and Seguin) Bergey et al.
Depositors H Lin, MS Nasoff, H Lin
Applications
Encodes the 68kD form of C. histolyticum collagenase, which may be useful in isolating islets from the human pancreas for clinical islet transplantation.
Insert
DNA: genomic
Insert lengths(kb): 1.700000047683716
Gene product: collagenase (68kD form)
Insert Size (kb) 1.700
Media Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--23.0, 4.4.
Encodes the 68kD form of C. histolyticum collagenase, which may be useful in isolating islets from the human pancreas for clinical islet transplantation.
References

Lin HC, Lei SP. Recombinant 68,000 dalton collagenase of Clostridium histolyticum. US Patent 5,418,157 dated May 23 1995

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