pADANS (68587)

Applications: YX-type (expression) shuttle vectorvector for constructing cDNA libraries  /  Depositors: Cold Spring Harbor Lab., MH Wigler, Cold Spring Harbor Lab.

Permits and Restrictions

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Designations pADANS
Depositors Cold Spring Harbor Lab., MH Wigler, Cold Spring Harbor Lab.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.8839998245239260
Vector: pADANS (plasmid)
Promoters: Promoter for expression ADH1
Construction: pADNS, ADH1 N-terminus
Construct size (kb): 7.883999824523926
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: ADH1
replicon: 2 micron
replicon: pMB1
MCS: HindIII...SfiI
transcription terminator: ADH1
coding sequence: ADH1 N-terminal 14 amino acids
YX-type (expression) shuttle vector
vector for constructing cDNA libraries
Yeast expression vector.
Recommendation for verification: EcoRV+HindIII--3.65, 3.5, 0.75; BglI--4.7, 3.2; EcoRI--4.2, 3.7.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Colicelli JJ, Wigler MH. Cloning by complementation and related processes. US Patent 5,527,896 dated Jun 18 1996

Colicelli J, et al. Expression of three mammalian cDNAs that interfere with RAS function in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 88: 2913-2917, 1991. PubMed: 1849280

Shipped frozen
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