pADANS (ATCC® 68587)

Applications: YX-type (expression) shuttle vectorvector for constructing cDNA libraries  /  Depositors: Cold Spring Harbor Lab., MH Wigler, Cold Spring Harbor Lab.

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Designations pADANS
Depositors Cold Spring Harbor Lab., MH Wigler, Cold Spring Harbor Lab.
Biosafety Level 1
Vector Information
Size (kb): 7.8839998245239260
Vector: pADANS (plasmid)
Promoters: Promoter for expression ADH1
Construction: pADNS, ADH1 N-terminus
Marker(s):LEU2,ampR
Construct size (kb): 7.883999824523926
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: ADH1
replicon: 2 micron
replicon: pMB1
MCS: HindIII...SfiI
transcription terminator: ADH1
coding sequence: ADH1 N-terminal 14 amino acids
Applications
YX-type (expression) shuttle vector
vector for constructing cDNA libraries
Comments
Yeast expression vector.
Recommendation for verification: EcoRV+HindIII--3.65, 3.5, 0.75; BglI--4.7, 3.2; EcoRI--4.2, 3.7.
Growth Conditions
Temperature: 37.0°C
References

Colicelli JJ, Wigler MH. Cloning by complementation and related processes. US Patent 5,527,896 dated Jun 18 1996

Colicelli J, et al. Expression of three mammalian cDNAs that interfere with RAS function in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 88: 2913-2917, 1991. PubMed: 1849280

Shipped frozen
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