p101JdeltaE5.0 [p101Jdelta1E5.0, p101delta1 5.0E] (ATCC® 67996)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: Temple University, CM Croce, Temple University

Permits and Restrictions
Permits and Restrictions

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Designations p101JdeltaE5.0 [p101Jdelta1E5.0, p101delta1 5.0E]
Species Homo sapiens, human
Depositors Temple University, CM Croce, Temple University
Applications
Has been used to detect t(10;14)(q24;q11) translocations associated with certain acute T-cell leukemias based on an alteration in the size of the BamHI fragment detected.
Vector
Construct size (kb): 7.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 5.0
Tissue: placenta
Gene product: T cell receptor, delta (V,D,J,C) [TCRD]
Insert Size (kb) 5.0
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.6; EcoRI--5.0, 2.8.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--1.1, 1.8, 3.0; HindIII--3.5, 4.0.
The MOLT-4 cell line (ATCC CRL-1582) is deleted for this fragment.
Detects the following human genomic germline restriction fragments (kb): BamHI--16.5; HindIII--5.8, 3.2.
Has been used to detect t(10;14)(q24;q11) translocations associated with certain acute T-cell leukemias based on an alteration in the size of the BamHI fragment detected.
Corresponds to the EcoRI fragment between Jdelta1 and Jdelta2.
References

Kagan J, et al. Clustering of breakpoints on chromosome 10 in acute T-cell leukemias with the t(10;14) chromosome translocation. Proc. Natl. Acad. Sci. USA 86: 4161-4165, 1989. PubMed: 2542965

Croce CM. Molecular probing for human T-cell leukemia and lymphoma. US Patent 5,198,338 dated Mar 30 1993

Croce CM. TCL-5 gene rearrangement involved in T-cell leukemia and melanoma. US Patent 5,242,795 dated Sep 7 1993

Product Sheet
Product Sheet
Product Sheet