pCLL7 (ATCC® 67448)

Applications: shuttle vector  /  Depositors: American Cyanamid Co., JS Feitelson, American Cyanamid Co.

Permits and Restrictions

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Designations pCLL7
Depositors American Cyanamid Co., JS Feitelson, American Cyanamid Co.
Biosafety Level 1
Vector Information
Size (kb): 8.3599996566772460
Vector: pCLL7 (plasmid)
Construction: pCLL4
Marker(s):ampR,tsrR
Construct size (kb): 8.359999656677246
Features: marker(s): ampR
marker(s): tsrR
replicon: pAkij1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 4.0; KpnI--8.5; PstI--8.5.
Cloning into the PstI site inactivates the ampicillin marker used for selection in Escherichia coli.
The pAkij1 ori may be useful in other taxa such as Bacillus, Staphylococcus and other Actinomycetes, including Streptosporangium, Actinoplanes, Nocardia, and Micromonospora.
Constructed by removing a 1.99 kb HindIII fragment from pCLL4 (ATCC 67447) and religating.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Feitelson JS. Multifunctional plasmid vectors from Actinomadura and Escherichia coli. US Patent 5,002,891 dated Mar 26 1991

Feitelson JS, et al. Genetic and biochemical characterization of the red gene cluster of streptomyces coelicolor A3(2). J. Gen. Microbiol. 131: 2431-2441, 1985. PubMed: 2999302

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet