Designation / Description PGP5-5 AND PTRX-2 PLASMIDS IN E COLI K38
U.S. Patent Number
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, uncut; HincII--3.4, 2.5, 1.25, 1.1, 0.72(doublet).
For pGP5-5, the insert size is 3.0 kb and the total size of the plasmid is 6.06 kb. For pTrx-2, the insert size is 0.47 kb per copy of the trxA gene and the total size of the plasmid is 3.23 kb.
pGP5-5 contains the replicon P15A. pTrx-2 contains the replicon ColE1.
T7 DNA polymerase consists of two subunits, a protein encoded by T7 gene 5 and Escherichia coli thioredoxin.
M13mGP1-2 (ATCC 40303) can coexist in the same cell as the two plasmids of ATCC 67287, pGP5-5 and pTrx-2. The T7 RNA polymerase of M13mGP1-2 can be used to regulate expression of T7 DNA polymerase by inducing the RNA polymerase with IPTG.
Escherichia coli K38 = HfrC lambda.
pTrx-2 contains two tandem copies of trxA cloned into ptac-12. Expression of the gene is controlled by the tac promoter and is inducible by IPTG. pTrx-2 is ampicillin resistant and contains a unique EcoRI site. It is also available as ATCC 67286.
pGP5-5 contains two segments of bacteriophage T7: nt 5667-6166 (phi1.1A and phi1.1B promoters) and nt 14306-16869 (gene 5). These are cloned into BamHI-HincII of pACYC177. pGP5-5 is kanamycin resistant and contains a unique XhoI site.
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Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
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Basic Documentation

Tabor S, Richardson CC. T7 DNA polymerase. US Patent 4,795,699 dated Jan 3 1989

Charles C Richardson, personal communication