pJP1 (ATCC® 67078)

Organism: Staphylococcus simulans Kloos and Schleifer  /  Clone Type: Clone  /  Depositors: Public Health Research Institution, PA Recsei, Public Health Research Institution

Designations pJP1
GenBank Number

M15686

Species Staphylococcus simulans Kloos and Schleifer
Depositors Public Health Research Institution, PA Recsei, Public Health Research Institution
Applications
produces protein lysostaphin
Vector
Construct size (kb): 8.0
Insert
DNA: genomic
Insert lengths(kb): 1.5
Gene product: lysostaphin
Target Gene: lysostaphin
Insert Size (kb) 1.5
Media Medium 2220: 1179 plus erythromycin (5 mcg/ml)
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--8.0; PstI--8.0; SphI--7.7, 0.3.
The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.43, 0.46, 0.50, 0.58, 0.62; SphI--1.23.
Constructed by excising the insert of pRG5 (ATCC 67076) with an EcoRI/HindIII digest, and blunt-ending the vector and insert with the Klenow fragment of DNA polymerase before ligation.
Contains the complete coding sequence (nt 245-1414 of the sequence record).
References

Recsei PA. Expression of the cloned lysostaphin gene. US Patent 4,931,390 dated Jun 5 1990

Recsei PA, et al. Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans. Proc. Natl. Acad. Sci. USA 84: 1127-1131, 1987. PubMed: 3547405

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