(67078)

U.S. Patent Number
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--8.0; PstI--8.0; SphI--7.7, 0.3.
The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.43, 0.46, 0.50, 0.58, 0.62; SphI--1.23.
Constructed by excising the insert of pRG5 (ATCC 67076) with an EcoRI/HindIII digest, and blunt-ending the vector and insert with the Klenow fragment of DNA polymerase before ligation.
Contains the complete coding sequence (nt 245-1414 of the sequence record).
Shipped freeze-dried
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Basic Documentation
References

Recsei PA. Expression of the cloned lysostaphin gene. US Patent 4,931,390 dated Jun 5 1990

Recsei PA, et al. Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans. Proc. Natl. Acad. Sci. USA 84: 1127-1131, 1987. PubMed: 3547405