HL-42 (ATCC® 65014)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: K Tryggvason

Designations HL-42
GenBank Number

J02778

Species Homo sapiens, human
Depositors K Tryggvason
Vector
Construct size (kb): 7.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 3.0
Tissue: placenta
Gene product: laminin, B1 polypeptide [LAMB1]
Alleles: A1, A2
Insert Size (kb) 3.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
The insert contains PstI sites at nucleotides 3460, 4327, and 4893 relative to the 5' end of the complete cDNA sequence.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 3.0; BamHI--7.4; PstI--4.4, 1.6, 0.8, 0.6; HindIII--7.4.
This insert contains the 3' sequences of the cDNA, from nucleotide 2413 to 5413.
HL-40 (ATCC 65008, 65009) and HL-42 (ATCC 65014, 65015) overlap.
Enzyme(s) not detecting polymorphism: EcoRI, SphI, BamHI, HindIII, MspI, TaqI, PstI, HaeIII, RsaI, HinfI (in 20 unrelated Finnish individuals).
References

Ikonen J, et al. A Hpa I polymorphism in the human laminin B1 chain gene on 7q22. Nucleic Acids Res. 17: 473, 1989. PubMed: 2563160

Pikkarainen T, et al. Human laminin B1 chain. A multidomain protein with gene (LAMB1) locus in the q22 region of chromosome 7. J. Biol. Chem. 262: 10454-10462, 1987. PubMed: 3611077

Karl Tryggvason, personal communication

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